RF-6000

RF-6000 spectrofluorometer — a combination of high accuracy and ease of use

The RF-6000 spectrofluorimeter is a Shimadzu fluorescence spectrophotometer that combines the achievements of modern technology and accumulated experience, characterized by maximum productivity in solving a wide range of analytical tasks. The new LabSolutions RF software provides convenient control of the instrument and has extensive analytical capabilities.

Features

  • The improved sensitivity and dynamic range of the spectrofluorimeter allow measurements of spectra not only of fluorescence, but also of bioluminescence, chemiluminescence and electroluminescence.
  • High scanning speed provides the possibility of quickly obtaining a spectrum in 3D format.
  • Correction function of excitation and emission spectra.
  • Measurements of fluorescence quantum yield and fluorescence quantum efficiency are available.
  • High sensitivity in its class: signal-to-noise ratio is more than 1000 (RMS) or more than 350 (peak-to-peak).
  • High scanning speed of 60000 nm/min allows to minimize analysis time.
  • Service life of the light source (xenon lamp) is about 2000 hours.
  • Extended spectral range up to 900 nm.
  • New LabSolutions RF software allows to significantly simplify analysis.
  • Validation procedures are included in the software.
  • The LabSolutions RF software status bar indicates the type of accessory used.
  • Large cuvette compartment for a wide range of analytical tasks.
  • Can be used to check/calibrate the wavelength scale in automatic mode.

LabSolutions RF Software

ПЗ спеціально розроблене для спрощення роботи з приладом. Весь шлях від вимірювання зразка до обробки отриманих даних максимально наочно представлений у вигляді функцій програмного забезпечення. Всі програми вимірювань мають одну і ту ж головну панель інструментів, деревоподібне меню і журнал подій, так що кожна програма може запускатися однаково. Для керування всіма спеціалізованими програмами флуоресцентного аналізу використовуються одні й ті ж операції. Вкладки для запуску режимів вимірювання, наприклад, спектрального та кількісного, зручно інтегровані в панель управління LabSolutions RF, що дозволяє легко вибрати потрібний режим. Крім цього, часто використовувані додатки Windows також можуть бути додані в панель управління LabSolutions RF.

  • Spectrum correction functions are included as standard and allow you to display edited spectra in real time. This allows for true excitation and fluorescence spectra to be obtained by correcting for instrument characteristics such as the emission characteristics of the light source and the spectral characteristics of the optical system. Thus, corrected spectra are obtained in real time. They can be easily compared with spectra measured using other instruments. The spectral correction functions were created using a calibrated standard light source and Shimadzu’s own correction methods. If an integrating sphere is used as an accessory, the spectral correction function is included as standard, eliminating the need to install a special light source.
  • High-speed 3D spectrum measurement. The dependence of the excitation wavelength on the fluorescence wavelength in the 3D spectrum is obtained by sequentially changing the excitation wavelength when measuring the fluorescence spectrum. 3D fluorescence spectra are useful for determining the optimal excitation and fluorescence wavelengths. The differences in the three-dimensional spectra in some cases allow for the identification of the source of the samples. Since the RF-6000 is capable of scanning samples at high speeds of up to 60,000 nm/min, 3D fluorescence spectra can be obtained quite quickly, even when measuring in the maximum wavelength range.
  • Conducting highly sensitive quantitative measurements. The use of fluorescence spectrophotometry for quantitative measurements is due to the high sensitivity of the method, as well as a wide range of linearity in several orders of magnitude. The software interface allows for the procedures for constructing calibration graphs to be carried out as simply and conveniently as possible for the user. Formulas can be entered into the calibration procedures for further recalculation of the measured value, which ultimately gives the user the required value without the need for recalculation in other programs.
  • Easy measurement of fluorescence quantum yield and quantum efficiency. The fluorescence quantum yield can be calculated by comparing the fluorescence spectra of the test samples with the results of a standard sample with a known quantum yield. An integrating sphere with a diameter of 100 mm is also used to calculate the fluorescence quantum efficiency. Thanks to the user-friendly design, the measurement and subsequent calculations of the quantum yield and fluorescence quantum efficiency are carried out at an intuitive level.